What are the differences of HPLC,UV
High-performance liquid chromatography
High-performance liquid chromatography (HPLC; formerly referred to as high-pressure liquid chromatography), is a technique in analytical chemistry used to separate, identify, and quantify each component in a mixture. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column.
HPLC has been used for manufacturing (e.g. during the production process of pharmaceutical and biological products), legal (e.g. detecting performance enhancement drugs in urine), research (e.g. separating the components of a complex biological sample, or of similar synthetic chemicals from each other), and medical (e.g. detecting vitamin D levels in blood serum) purposes
Most of time ,HPLC can be used to identify herbal extract.
UV/VIS spectroscopy is widely used as a technique in chemistry to analyze chemical structure, the most notable one being conjugated systems. UV radiation is often used to excite a given sample where the fluorescent emission is measured with a spectrofluorometer. In biological research, UV light is used for quantification of nucleic acids or proteins.
A collection of mineral samples brilliantly fluorescing at various wavelengths as seen while being irradiated by UV light.
Ultraviolet lamps are also used in analyzing minerals and gems.
In pollution control applications, ultraviolet analyzers are used to detect emissions of nitrogen oxides, sulfur compounds, mercury and ammonia, for example in the flue gas of fossil fired power plants. Ultraviolet light can detect thin sheens of spilled oil on water, either by the high reflectivity of oil films at UV wavelengths, fluorescence of compounds in oil, or by absorbing of UV light created by Raman scattering in water.
In herbal ingredients field,we use this method to test percentage of active ingredient in plant extract.